Analisis In Silico Senyawa Kimia dari Ekstrak Daun Sukun (Artocarpus altilis) terhadap PPAR-γ dan Uji In Vivo pada Ikan Zebra (Danio rerio) yang Berpotensi sebagai Antidiabetes

Abstract

Background: Diabetes mellitus is a chronic disease that occurs when the pancreas does not produce enough insulin or when the body cannot use the insulin produced effectively. Breadfruit leaves are used as antidiabetics in the presence of bioactive components such as flavonoids. One of the receptors that play a role in the treatment of diabetes is PPAR-γ. Objective: To determine the secondary metabolites of ethanol breadfruit leaves extract of against PPAR-γ receptors that have potential as antidiabetics in silico and in vivo. Methods: The stages of this study include LC-HRMS/MS analysis, in silico testing includes prediction of biological activity (PASS online), physicochemistry (Drug design Lipinski), pharmacokinetics (SwissADME), toxicity (ProTox-II) and molecular docking (Autodock tools and Autodock vina), in vivo testing used animals (zebrafish), with parameters fasting blood glucose levels, and data analyzed using One way ANOVA. Results: In silico, the chemical compounds of breadfruit leaves that have interactions with PPAR-γ receptors by looking at the low docking score and have the same amino acid residues as the comparator are Apigenin-7-o-glucoronide, Tiliroside, Phloretin, and Artocarpin. In vivo, ethanol extract of breadfruit leaves has antidiabetic activity to reduce blood glucose levels of zebrafish, while maintaining the number of islets of Langerhans at doses of 200, 300, and 400 mg. Conclusion: From the results of this study, it is known that ethanol extract of breadfruit leaves has activity as an antidiabetic proven in silico and in vivo.