Pengaruh Ekstrak Sereh Wangi (Cymbopogon nardus) sebagai Alternatif Bahan Cavity Cleanser terhadap Pertumbuhan Bakteri Streptococcus sanguinis (in Vitro)

Abstract

The ideal cavity cleanser must have a low level of toxicity, be biocompatible, antibacterial, and be able to remove the smear layer. This study aims to determine the effect of citronella extract (cymbopogun nardus) as an alternative to Cavity cleanser on the growth of streptococcus sanguinis bacteria by looking for KHM and KBM values. Laboratory experimental research with a post test only control group design using Streptococcus blood ATCC 10551 samples. The KHM and KBM values are determined by the Misra Drop Plates Miles method. Citronella extract was diluted with MHB through the dilution method, so that 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% were obtained with 4 replications in each treatment group including Chlorhexidine digluconate 2%, after which it was filled with 0.1 ml of bacteria, then homogenized and incubated for 48 hours at 37°C. After that, take 0.1 ml of the solution from the dilution procedure and then replant it into Mueller Hinton agar medium and then incubate. The results of the Anova test showed that all concentrations of citronella extract had the effect of killing Streptococcus blood bacteria (p<0.05). That this citronella extract at a concentration of 6.25% to 100% has an effect on the growth of Streptococcus blood bacteria. The LSD test results showed that the KBM value of citronella extract was 6.25% and KHM 3.125%.