Isolasi dan Karakterisasi Bakteri Asam Laktat pada Minuman Fermentasi Nira Nipah

Abstract

Background: The nipah plant is a plant of the family that usually grows in mangrove coastal areas. Nira nipah contains several types of microorganisms including lactic acid bacteria. However, its character and species are not yet known. This information is important for the development of lactic acid bacteria and nipah sap as probiotics. Objective: This research is a qualitative descriptive study that aims to determine characteristics of lactic acid bacteria from nira nipah fermentation. Observation of colony morphology bacteria can be done after obtaining pure isolation from samples following with identification and characterization of lactic acid bacteria, morphological test, Gram painting, catalase test, triple sugar iron (TSI) test, fermentation, temperature resistance, sulfur indole motility (SIM), Simmon citrate, and coagulation. Result: It is known that 14 colonies of bacterial isolates obtained from the nira nipah, 10 of the 14 bacterial isolates identified are lactic acid bacteria while the other 4 colonies of bacterial isolates are identified as not lactic acid bacteria. Conclusiont: The identified lactic acid bacteria are bacillus, with a milky white color, Gram positive bacteria, have negative catalase and coagulase, has a heterofermentation type of fermentation, can withstand and multiply in salt concentrations of 5 %, 6.5 %, 10 %, have resistance to temperatures in the range of 14 °C and 37 °C, have a negative Sulfur Indole Motility (SIM) test, have a triple sugar iron (TSI) acid/acid (A/A) test, Simmon citrate negative, and coagulation negative. These characteristics are species of lactic acid bacteria.