Ekstrak Kulit Durian (Durio Zibethinus Murr.) sebagai Renoprotector pada Tikus (Rattus Norvegicus) yang di Induksi 7,12-Dimetilbenz-[Α] Antrasena (DMBA) Ekstrak

Abstract

Durian fruit peel extract (Durio zibethinus Murr.) contains bioactive compound terpenoids, flavonoids, phenols and alkaloids. These bioactive compounds can be chemopreventive agents to prevent cancer development, to inhibit, to neutralize carcinogens, to improve the body's immune system, and to repair cell damage in organ tissues exposed of carcinogens. This study aims to determine the durian skin extract (Durio zibethinus Murr.) as a renal protector in rats (Rattus norvegicus) induced by 7,12 Dimethylbenz-[A] Anthracene (DMBA). This study consists of six treatments using a completely randomized design (CRD), which are negative control (only given feed ad libitum); positive control (induced DMBA 10 mg/kgBW); DMBA induction + methotrexate 9 mg/kgBW; and DMBA induction + durian peel extract at doses of 150, 300 and 450mg/kgBW. The preparation of kidney organs is made histologically by paraffin method and Hematoxylin-Eosin (HE) staining. The results of the research show the durian peel extract does not affect kidney morphology (ex: the colour, the weight and the volume), however it is potentially improving kidney histology (p<0.05) including the glomerular area, the narrowing of proximal tubules and necrosis. Durian peel extract has the potential as an effective renal protector at a dose of 450 mg/kgBW.