| dc.description.abstract | Surrounding the waters of South Nias are two large bodies of water, namely
the Andaman Sea and the Malacca Strait. Differences in current patterns expand
the distribution range and coupled with differences in geographical location cause
more variation in a species. The increasing demand for Caranx type fish on the
market makes these fish vulnerable to extinction if not managed properly, so
molecular scientific data is needed for fisheries management. The aim of this
research is to look at the genetic pattern of Carangidae from Nias waters using the
COI mitochondrial DNA Barcoding gene. This research was conducted from
October to November 2023 and samples were taken from several adjacent steps.
The extraction method was carried out using a modified Chelex protocol. In this
study, 8 sequences were produced. In the phylogenetic tree, C.sexfasciatus is
divided into 4 different haplotypes, combined into 4 different clades due to the
distribution of matings of the same species in the population, as evidenced by the
presence of the same haplotype at the location of the nest. The diversity values of 8
sample sequences are combined into 2 large clades and have an accuracy of 99%
between other C.sexfasciatus clades forming 7 subclades of species Caranx
sexfasciatus, Caranx ignobilis, and Carangodes ferdau which have varying
bootstrap values of 52%, 57%, 64%, 83%, 100%. The farthest nucleotide distance
between samples and collectors C.sexfas3 and C.sexfas4 is 0.0063 different
haplotype OQ387298, and the closest is C.sexfas1 and C.sexfas2 with the species
C.sexfas5 subcalde nearby, and the value of the farthest genetic distance varies
between genera in C ignobilis with Carangoides ferdau and shows that these three
species are still closely related. | en_US |
