Uji Aktivitas Antioksidan dari Ekstrak Etanol Daun Katuk (Sauropus androgynus L. Merr) dengan Metode DPPH

Abstract

Background: Free radicals cause premature aging, therefore antioxidants are needed to prevent premature aging. Katuk leaves (Sauropus androgynus (L.) Merr.) have antioxidant activity because they contain flavonoid compounds, namely kaempferol, where flavonoid compounds can act as antioxidants. Katuk (Sauropus androgynus L. Merr) is a plant belonging to the Euphorbiaceae family. Katuk leaves contain many antioxidants such as flavonoids, xanthones, triterpenoids and stilbene. Of the several antioxidants above, the antioxidants that have been most researched are flavonoids which have anti-inflammatory activity. Testing for antioxidant activity can be done in vitro using the DPPH method. DPPH (1,1 diphenyl-2picrylhydrazyl) is a stable radical compound. DPPH is used to research antioxidant activity through its ability to capture free radicals. Objective: Testing the antioxidant activity of the ethanol extract of katuk leaves was carried out using the DPPH method. Results: Katuk leaf ethanol extract has antioxidant activity IC50 of 110.1652 μg/ml with a regression equation Y = 0.4722 x – 2.02 and a correlation coefficient of 0.9897. Conclusion: the IC50 antioxidant activity obtained from the ethanol extract of katuk leaves was 110.1652 μg/ml which was categorized as medium.