Aktivitas Antioksidan Ekstrak Hidroalkohol Kulit Batang Attarasa (Litsea Cubeba Lour.) dengan Metode Cuprac (Cupric Ion Reducing Antioxidant Capacity)

Abstract

Background: Oxidative stress is an imbalance between free radicals and antioxidants. Oxidative stress can lead to accelerated aging and the emergence of various degenerative diseases such as cataracts, rheumatism, coronary heart disease, liver and cancer. Antioxidants are agents that can be used to prevent and repair damage in the body due to free radicals. One type of spice that has the potential to be developed is attarasa (Litsea cubeba Lour.). Attarasa stem bark contains alkaloids, flavonoids, saponins, tannins, glycosides and steroids which have the potential as natural antioxidants. Objective: The purpose of this research is to determine value of antioxidant activity and the effect of different concentrations of alcohol solvents during extraction on the value of the antioxidant activity of the hydroalcoholic extract of attarasa stem bark. Methods: The research was conducted experimentally including the manufacture of dried powder, characterization of dried powder, extraction by maceration method, , identification of secondary metabolites in extracts. Furthermore, the antioxidant activity was tested using the CUPRAC method and analysis of IC50 (Inhibition Concentration) values. Result: Characterization of dried powder of attarasa stem bark obtained water content was 3.96%, water-soluble extract content was 17.10%, ethanol-soluble extract content was 9.63%, total ash content was 2.23%, and acid insoluble ash content was 0.32%. The antioxidant activity of each attarasa stem bark extract has an IC50 value using the CUPRAC method. 50% hydroalcoholic extract (22.95 ± 0.01 μg/mL), 60% hydroalcoholic extract (27.04 ± 0.08 μg/mL), 70% hydroalcoholic extract (26.72 ± 0.11 μg/mL) , 80% hydroalcoholic extract (28.72 ± 0.32 μg/mL), and 90% hydroalcoholic extract (28.43 ± 0.26 μg/mL) and quercetin as comparison standard (2.25 ± 0.01 μg/ ml). Conclusion: The results of this study can be concluded that the antioxidant activity of variations of the hydroalcoholic extract of attarasa stem bark has a very strong antioxidant activity (IC50 < 50 μg/mL ) and 50% hydroalcohol extract of attarasa stem bark had the highest antioxidant activity.