Perbedaan Sitotoksisitas Bahan Irigasi Saluran Akar Gigi QDs Ekstrak Lerak (Sapindus rarak DC) dengan Kombinasi NaOCl 2,5% dan EDTA 17% Terhadap Pertumbuhan Sel Fibroblas 3T3 (In vitro)

Abstract

Root canal cleaning and disinfection are highly dependent on irrigation materials, especially due to the complex anatomy of the canal. One type of material that has the potential to be a root canal irrigation material is Quantum dots of extract lerak. Before being used as an alternative root canal irrigation material, it is necessary to know the characteristics of the material whether it is biocompatible enough by testing its cytotoxicity. The purpose of this study was to determine the cytotoxicity of QDs lerak extract and its differences with the combination of 2.5% NaOCl + 17% EDTA against 3T3 fibroblast cells using the MTT test. This study used a post-test only control group design. Consisting of eight groups, QDs lerak extract 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.625%, and NaOCl 2.5% + EDTA 17%. Cell cytotoxicity testing was carried out by observing the viability of 3T3 fibroblast cells that had been given the test solution, then given MTT solution and the absorbance reading was carried out with a microplate (ELISA) reader after a 24-hour incubation period. The obtained data were analyzed using One-way ANOVA followed by Games Howell test. The test results that all groups showed a p-value = 0.00 (p <0.05), which means that statistically there was a significant difference in the average value and standard deviation of all observation groups. The results showed that QDs lerak extract 100%, 25%, 12.5%, 6.25%, 3.125%, 1.625%, and NaOCl 2.5% + EDTA 17% were not toxic to 3T3 fibroblast cells within 24 hours. The concentration that can be used as an alternative root canal irrigation material is QDs lerak extract 6.25% because it has the highest viability value.